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Real Time PCR

 

Lab PCR / Lab Evaporators

Laboratory PCR Laboratory PCR MG 48+ Gradinet PCR
PCR Thermal Cycle MG 96+ Gradinet Thermal Cycler PCR Real Time PCR
Digital Rotary Evaporator Vertical Type

We are manufacturer, supplier and exporter of world class JLS India PCR Equipment, Laboratory PCR MG 48+, Gradinet PCR, PCR Thermal Cycle MG 96+, Gradinet Thermal Cycler PCR, Real Time PCR, Digital Rotary Evaporator Vertical Type . Our technologically advanced range of products are capable of providing even the most minute findings and observations to the user. We are a team of experienced technocrats and scientists to design the world's best range of products for our valued clients. By leveraging our full potentials, we are capable of designing tailor made products as per the exact requirements of the clients. We are accredited with ISO 9001, CE and EU certification.

real-time-pcr-machine

JLS India Advance Technology GMI Design Fully Automatic Vertical Autoclave.

Real Time PCR

We are manufacturer, supplier and exporter of world class JLS India PCR Equipment, Laboratory PCR MG 48+, Gradinet PCR, PCR Thermal Cycle MG 96+, Gradinet Thermal Cycler PCR, Real Time PCR, Digital Rotary Evaporator Vertical Type . Our technologically advanced range of products are capable of providing even the most minute findings and observations to the user. We are a team of experienced technocrats and scientists to design the world's best range of products for our valued clients. By leveraging our full potentials, we are capable of designing tailor made products as per the exact requirements of the clients. We are accredited with ISO 9001, CE and EU certification.

Real Time PCR procedure

In a real time PCR protocol, a fluorescent reporter molecule is used to monitor the PCR as it progresses. The fluorescence emitted by the reporter molecule manifolds as the PCR product accumulates with each cycle of amplification. Based on the molecule used for the detection, the real time PCR techniques can be categorically placed under two heads:

Limitations of End-point PCR

In a PCR reaction as the reaction progresses, the reagents are being consumed as a result of amplification. Now the PCR product is no longer being doubled at each cycle due to this reagent constraint. This depletion will occur at different rates for each replicate. Thus, the samples begin to diverge in their quantities. This diminished amplification is the linear phase of the reaction. The plateau for each tube will differ due to the different reaction kinetics for each sample. It is in this phase where traditional PCR takes its measurement, also known as the end-point. This End-Point Detection has some problems such as low resolution, poor precision, low sensitivity and the need for post PCR processing. Also, the results of this detection are not expressed in numbers and there is no scope for automation.

Real Time PCR Applications Include

  • Quantitative MRNA expression studies.
  • DNA copy number measurements in genomic or viral DNAs.
  • Allelic discrimination assays or SNP genotyping.
  • Verification of microarray results.
  • Drug therapy efficacy.
  • DNA damage measurement.

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